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Promega prl-tk e2241
Prl Tk E2241, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-tk+e2241/pgl3+basic/pmc11711668-226-23-25
Average 90 stars, based on 1 article reviews
prl-tk e2241 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Transfection:

Article Title: ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis.
Article Snippet: H9C2 cells were infected with ATF4-overexpressing adenovirus followed the above-mentioned steps, 24 h after SOR treatment, luciferase reporter plasmid, and pRL-TK (E2241, Promega, Madison, WI, USA) were transfected into cells.

Article Title: Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2
Article Snippet: Next, 450 ng pCAG-human n SREBP2 or a control plasmid, pCAG-Cre, was co-transfected with 500 ng reporter plasmids and 100 ng pRL-TK (Promega, E2241).

Article Title: ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1
Article Snippet: Each plasmid was transfected with 500 ng recombinant constructs, together with 50 ng per well of pRL-TK (E2241, Promega).

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [ ], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis.
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [33], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Reduction of specific enterocytes from loss of intestinal LGR4 improves lipid metabolism in mice
Article Snippet: Cotransfected the plasmids together with pRL-TK (E2241, Promega, USA) into 293T cells.

Article Title: SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microglia
Article Snippet: BV2 cells were transfected with 0.5 μg of pGL4 plasmids with wild-type VEGF-C promoter (pGL4-VEGF-C-WT), pGL4 plasmids with mutant VEGF-C promoter (pGL4-VEGF-C-MUT), or pRL-TK (E2241, Promega, USA), which was used as an internal control.

Article Title: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
Article Snippet: Sixteen hours later, cells were co-transfected with 15 fmol pRL-TK (Promega, E2241) and 150 fmol of either pGL4.27[luc2P/minP/Hygro] empty vector or pGL4.27[luc2P/minP/Hygro] containing either eSOX17 , eSOX17.1 or eSOX17.2 utilizing Lipofectamin Stem Transfection Reagent (Thermo Fisher Scientific, STEM00003) following the manufacturer’s instructions.

Mutagenesis:

Article Title: ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis.
Article Snippet: H9C2 cells were infected with ATF4-overexpressing adenovirus followed the above-mentioned steps, 24 h after SOR treatment, luciferase reporter plasmid, and pRL-TK (E2241, Promega, Madison, WI, USA) were transfected into cells.

Article Title: Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2
Article Snippet: Next, 450 ng pCAG-human n SREBP2 or a control plasmid, pCAG-Cre, was co-transfected with 500 ng reporter plasmids and 100 ng pRL-TK (Promega, E2241).

Article Title: ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1
Article Snippet: Each plasmid was transfected with 500 ng recombinant constructs, together with 50 ng per well of pRL-TK (E2241, Promega).

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [ ], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis.
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [33], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Reduction of specific enterocytes from loss of intestinal LGR4 improves lipid metabolism in mice
Article Snippet: Cotransfected the plasmids together with pRL-TK (E2241, Promega, USA) into 293T cells.

Article Title: SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microglia
Article Snippet: BV2 cells were transfected with 0.5 μg of pGL4 plasmids with wild-type VEGF-C promoter (pGL4-VEGF-C-WT), pGL4 plasmids with mutant VEGF-C promoter (pGL4-VEGF-C-MUT), or pRL-TK (E2241, Promega, USA), which was used as an internal control.

Article Title: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
Article Snippet: Sixteen hours later, cells were co-transfected with 15 fmol pRL-TK (Promega, E2241) and 150 fmol of either pGL4.27[luc2P/minP/Hygro] empty vector or pGL4.27[luc2P/minP/Hygro] containing either eSOX17 , eSOX17.1 or eSOX17.2 utilizing Lipofectamin Stem Transfection Reagent (Thermo Fisher Scientific, STEM00003) following the manufacturer’s instructions.

Control:

Article Title: ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis.
Article Snippet: H9C2 cells were infected with ATF4-overexpressing adenovirus followed the above-mentioned steps, 24 h after SOR treatment, luciferase reporter plasmid, and pRL-TK (E2241, Promega, Madison, WI, USA) were transfected into cells.

Article Title: Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2
Article Snippet: Next, 450 ng pCAG-human n SREBP2 or a control plasmid, pCAG-Cre, was co-transfected with 500 ng reporter plasmids and 100 ng pRL-TK (Promega, E2241).

Article Title: ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1
Article Snippet: Each plasmid was transfected with 500 ng recombinant constructs, together with 50 ng per well of pRL-TK (E2241, Promega).

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [ ], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis.
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [33], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Reduction of specific enterocytes from loss of intestinal LGR4 improves lipid metabolism in mice
Article Snippet: Cotransfected the plasmids together with pRL-TK (E2241, Promega, USA) into 293T cells.

Article Title: SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microglia
Article Snippet: BV2 cells were transfected with 0.5 μg of pGL4 plasmids with wild-type VEGF-C promoter (pGL4-VEGF-C-WT), pGL4 plasmids with mutant VEGF-C promoter (pGL4-VEGF-C-MUT), or pRL-TK (E2241, Promega, USA), which was used as an internal control.

Article Title: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
Article Snippet: Sixteen hours later, cells were co-transfected with 15 fmol pRL-TK (Promega, E2241) and 150 fmol of either pGL4.27[luc2P/minP/Hygro] empty vector or pGL4.27[luc2P/minP/Hygro] containing either eSOX17 , eSOX17.1 or eSOX17.2 utilizing Lipofectamin Stem Transfection Reagent (Thermo Fisher Scientific, STEM00003) following the manufacturer’s instructions.

Plasmid Preparation:

Article Title: ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis.
Article Snippet: H9C2 cells were infected with ATF4-overexpressing adenovirus followed the above-mentioned steps, 24 h after SOR treatment, luciferase reporter plasmid, and pRL-TK (E2241, Promega, Madison, WI, USA) were transfected into cells.

Article Title: Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2
Article Snippet: Next, 450 ng pCAG-human n SREBP2 or a control plasmid, pCAG-Cre, was co-transfected with 500 ng reporter plasmids and 100 ng pRL-TK (Promega, E2241).

Article Title: ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1
Article Snippet: Each plasmid was transfected with 500 ng recombinant constructs, together with 50 ng per well of pRL-TK (E2241, Promega).

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [ ], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis.
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [33], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Reduction of specific enterocytes from loss of intestinal LGR4 improves lipid metabolism in mice
Article Snippet: Cotransfected the plasmids together with pRL-TK (E2241, Promega, USA) into 293T cells.

Article Title: SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microglia
Article Snippet: BV2 cells were transfected with 0.5 μg of pGL4 plasmids with wild-type VEGF-C promoter (pGL4-VEGF-C-WT), pGL4 plasmids with mutant VEGF-C promoter (pGL4-VEGF-C-MUT), or pRL-TK (E2241, Promega, USA), which was used as an internal control.

Article Title: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
Article Snippet: Sixteen hours later, cells were co-transfected with 15 fmol pRL-TK (Promega, E2241) and 150 fmol of either pGL4.27[luc2P/minP/Hygro] empty vector or pGL4.27[luc2P/minP/Hygro] containing either eSOX17 , eSOX17.1 or eSOX17.2 utilizing Lipofectamin Stem Transfection Reagent (Thermo Fisher Scientific, STEM00003) following the manufacturer’s instructions.

Recombinant:

Article Title: ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis.
Article Snippet: H9C2 cells were infected with ATF4-overexpressing adenovirus followed the above-mentioned steps, 24 h after SOR treatment, luciferase reporter plasmid, and pRL-TK (E2241, Promega, Madison, WI, USA) were transfected into cells.

Article Title: Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2
Article Snippet: Next, 450 ng pCAG-human n SREBP2 or a control plasmid, pCAG-Cre, was co-transfected with 500 ng reporter plasmids and 100 ng pRL-TK (Promega, E2241).

Article Title: ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1
Article Snippet: Each plasmid was transfected with 500 ng recombinant constructs, together with 50 ng per well of pRL-TK (E2241, Promega).

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [ ], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis.
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [33], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Reduction of specific enterocytes from loss of intestinal LGR4 improves lipid metabolism in mice
Article Snippet: Cotransfected the plasmids together with pRL-TK (E2241, Promega, USA) into 293T cells.

Article Title: SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microglia
Article Snippet: BV2 cells were transfected with 0.5 μg of pGL4 plasmids with wild-type VEGF-C promoter (pGL4-VEGF-C-WT), pGL4 plasmids with mutant VEGF-C promoter (pGL4-VEGF-C-MUT), or pRL-TK (E2241, Promega, USA), which was used as an internal control.

Article Title: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
Article Snippet: Sixteen hours later, cells were co-transfected with 15 fmol pRL-TK (Promega, E2241) and 150 fmol of either pGL4.27[luc2P/minP/Hygro] empty vector or pGL4.27[luc2P/minP/Hygro] containing either eSOX17 , eSOX17.1 or eSOX17.2 utilizing Lipofectamin Stem Transfection Reagent (Thermo Fisher Scientific, STEM00003) following the manufacturer’s instructions.

Construct:

Article Title: ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis.
Article Snippet: H9C2 cells were infected with ATF4-overexpressing adenovirus followed the above-mentioned steps, 24 h after SOR treatment, luciferase reporter plasmid, and pRL-TK (E2241, Promega, Madison, WI, USA) were transfected into cells.

Article Title: Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2
Article Snippet: Next, 450 ng pCAG-human n SREBP2 or a control plasmid, pCAG-Cre, was co-transfected with 500 ng reporter plasmids and 100 ng pRL-TK (Promega, E2241).

Article Title: ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1
Article Snippet: Each plasmid was transfected with 500 ng recombinant constructs, together with 50 ng per well of pRL-TK (E2241, Promega).

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [ ], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis.
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [33], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Reduction of specific enterocytes from loss of intestinal LGR4 improves lipid metabolism in mice
Article Snippet: Cotransfected the plasmids together with pRL-TK (E2241, Promega, USA) into 293T cells.

Article Title: SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microglia
Article Snippet: BV2 cells were transfected with 0.5 μg of pGL4 plasmids with wild-type VEGF-C promoter (pGL4-VEGF-C-WT), pGL4 plasmids with mutant VEGF-C promoter (pGL4-VEGF-C-MUT), or pRL-TK (E2241, Promega, USA), which was used as an internal control.

Article Title: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
Article Snippet: Sixteen hours later, cells were co-transfected with 15 fmol pRL-TK (Promega, E2241) and 150 fmol of either pGL4.27[luc2P/minP/Hygro] empty vector or pGL4.27[luc2P/minP/Hygro] containing either eSOX17 , eSOX17.1 or eSOX17.2 utilizing Lipofectamin Stem Transfection Reagent (Thermo Fisher Scientific, STEM00003) following the manufacturer’s instructions.

Positive Control:

Article Title: ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis.
Article Snippet: H9C2 cells were infected with ATF4-overexpressing adenovirus followed the above-mentioned steps, 24 h after SOR treatment, luciferase reporter plasmid, and pRL-TK (E2241, Promega, Madison, WI, USA) were transfected into cells.

Article Title: Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2
Article Snippet: Next, 450 ng pCAG-human n SREBP2 or a control plasmid, pCAG-Cre, was co-transfected with 500 ng reporter plasmids and 100 ng pRL-TK (Promega, E2241).

Article Title: ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1
Article Snippet: Each plasmid was transfected with 500 ng recombinant constructs, together with 50 ng per well of pRL-TK (E2241, Promega).

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [ ], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis.
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [33], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Reduction of specific enterocytes from loss of intestinal LGR4 improves lipid metabolism in mice
Article Snippet: Cotransfected the plasmids together with pRL-TK (E2241, Promega, USA) into 293T cells.

Article Title: SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microglia
Article Snippet: BV2 cells were transfected with 0.5 μg of pGL4 plasmids with wild-type VEGF-C promoter (pGL4-VEGF-C-WT), pGL4 plasmids with mutant VEGF-C promoter (pGL4-VEGF-C-MUT), or pRL-TK (E2241, Promega, USA), which was used as an internal control.

Article Title: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
Article Snippet: Sixteen hours later, cells were co-transfected with 15 fmol pRL-TK (Promega, E2241) and 150 fmol of either pGL4.27[luc2P/minP/Hygro] empty vector or pGL4.27[luc2P/minP/Hygro] containing either eSOX17 , eSOX17.1 or eSOX17.2 utilizing Lipofectamin Stem Transfection Reagent (Thermo Fisher Scientific, STEM00003) following the manufacturer’s instructions.

Infection:

Article Title: ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis.
Article Snippet: H9C2 cells were infected with ATF4-overexpressing adenovirus followed the above-mentioned steps, 24 h after SOR treatment, luciferase reporter plasmid, and pRL-TK (E2241, Promega, Madison, WI, USA) were transfected into cells.

Article Title: Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2
Article Snippet: Next, 450 ng pCAG-human n SREBP2 or a control plasmid, pCAG-Cre, was co-transfected with 500 ng reporter plasmids and 100 ng pRL-TK (Promega, E2241).

Article Title: ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1
Article Snippet: Each plasmid was transfected with 500 ng recombinant constructs, together with 50 ng per well of pRL-TK (E2241, Promega).

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [ ], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis.
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [33], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Reduction of specific enterocytes from loss of intestinal LGR4 improves lipid metabolism in mice
Article Snippet: Cotransfected the plasmids together with pRL-TK (E2241, Promega, USA) into 293T cells.

Article Title: SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microglia
Article Snippet: BV2 cells were transfected with 0.5 μg of pGL4 plasmids with wild-type VEGF-C promoter (pGL4-VEGF-C-WT), pGL4 plasmids with mutant VEGF-C promoter (pGL4-VEGF-C-MUT), or pRL-TK (E2241, Promega, USA), which was used as an internal control.

Article Title: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
Article Snippet: Sixteen hours later, cells were co-transfected with 15 fmol pRL-TK (Promega, E2241) and 150 fmol of either pGL4.27[luc2P/minP/Hygro] empty vector or pGL4.27[luc2P/minP/Hygro] containing either eSOX17 , eSOX17.1 or eSOX17.2 utilizing Lipofectamin Stem Transfection Reagent (Thermo Fisher Scientific, STEM00003) following the manufacturer’s instructions.

Luciferase:

Article Title: ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis.
Article Snippet: H9C2 cells were infected with ATF4-overexpressing adenovirus followed the above-mentioned steps, 24 h after SOR treatment, luciferase reporter plasmid, and pRL-TK (E2241, Promega, Madison, WI, USA) were transfected into cells.

Article Title: Postnatal eye size in mice is controlled by SREBP2-mediated transcriptional repression of Lrp2 and Bmp2
Article Snippet: Next, 450 ng pCAG-human n SREBP2 or a control plasmid, pCAG-Cre, was co-transfected with 500 ng reporter plasmids and 100 ng pRL-TK (Promega, E2241).

Article Title: ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1
Article Snippet: Each plasmid was transfected with 500 ng recombinant constructs, together with 50 ng per well of pRL-TK (E2241, Promega).

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [ ], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Krüppel-like factor 9 (KLF9) links hormone dysregulation and circadian disruption to breast cancer pathogenesis.
Article Snippet: Cells were then transfected in quadruplicates, with 475 ng pGL4.23-eKSM/aKSM/KDE or the positive control pGL4.23-ERRFI1 downstream enhancer (EDE) [33], and 25 ng pRL-TK (Promega, E2241) as a normalization control using the XtremeGENE HP DNA Transfection Reagent (Roche, 6366236001) as per the manufacturer’s protocol.

Article Title: Reduction of specific enterocytes from loss of intestinal LGR4 improves lipid metabolism in mice
Article Snippet: Cotransfected the plasmids together with pRL-TK (E2241, Promega, USA) into 293T cells.

Article Title: SIX1 aggravates the progression of spinal cord injury in mice by promoting M1 polarization of microglia
Article Snippet: BV2 cells were transfected with 0.5 μg of pGL4 plasmids with wild-type VEGF-C promoter (pGL4-VEGF-C-WT), pGL4 plasmids with mutant VEGF-C promoter (pGL4-VEGF-C-MUT), or pRL-TK (E2241, Promega, USA), which was used as an internal control.

Article Title: T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
Article Snippet: Sixteen hours later, cells were co-transfected with 15 fmol pRL-TK (Promega, E2241) and 150 fmol of either pGL4.27[luc2P/minP/Hygro] empty vector or pGL4.27[luc2P/minP/Hygro] containing either eSOX17 , eSOX17.1 or eSOX17.2 utilizing Lipofectamin Stem Transfection Reagent (Thermo Fisher Scientific, STEM00003) following the manufacturer’s instructions.



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Promega prl-tk control plasmid e2241
Prl Tk Control Plasmid E2241, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-tk+e2241/pgl3+basic/pmc11666238-360-18-22
Average 90 stars, based on 1 article reviews
prl-tk control plasmid e2241 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega prl-tk plasmid e2241
Prl Tk Plasmid E2241, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-tk+e2241/pgl3+basic/10__26599_slash_fshw__2024__9250347-46-30-33
Average 90 stars, based on 1 article reviews
prl-tk plasmid e2241 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega prl-tk renilla luciferase reporter plasmid e2241
Prl Tk Renilla Luciferase Reporter Plasmid E2241, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-tk+e2241/pgl3+basic/pmc11189974-77-7-13
Average 90 stars, based on 1 article reviews
prl-tk renilla luciferase reporter plasmid e2241 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega prl-tk (e2241) plasmid
Prl Tk (E2241) Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-tk+e2241/pgl3+basic/pmc10981688-328-20-26
Average 90 stars, based on 1 article reviews
prl-tk (e2241) plasmid - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega tk promoter- renilla luciferase construct (prl-tk, #e2241)
Tk Promoter Renilla Luciferase Construct (Prl Tk, #E2241), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-tk+e2241/pgl3+basic/pmc10880820-111-3-13
Average 90 stars, based on 1 article reviews
tk promoter- renilla luciferase construct (prl-tk, #e2241) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Promega prl-tk plasmid (e2241)
Prl Tk Plasmid (E2241), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-tk+e2241/pgl3+basic/pm37819133-218-11-17
Average 90 stars, based on 1 article reviews
prl-tk plasmid (e2241) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

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